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Patent Pending PROBLEM TO BE SOLVED: To provide a method of detection enabling the discrimination of a mutation lineage in a crop such as rice, etc., accurately and simply in an optional stage in the circulation market of the crops, and also enabling the discrimination of agricultural crops between specific places of production (e.g. the discrimination between the agricultural crops produced in a specific prefecture and in another prefecture or discrimination between a cultivated area of an agricultural crop and another cultivated area).