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Patent Pending In enzymology, an aminoacylase (EC 3.5.1.14) is an enzyme that catalyzes the chemical reaction N-acyl-L-amino acid + H2O \rightleftharpoons carboxylate + L-amino acid Mechanism Summary rightleft.png Thus, the two substrates of this enzyme are N-acyl-L-amino acid and H2O, whereas its two products are carboxylate and L-amino acid.