Positional sequencing by hybridization

Patent Pending Positional Sequencing is a method of sequencing DNA that simultaneously generates information about both identity and location of nucleotide sequences.[1] The method involves detecting the location of sequence specific recognition events (e.g., such as hybridization of probes of known sequence) on single DNA molecules in each read, and generating maps of the location of such events. Multiple reads can be assembled into a consensus map that identifies the multiple locations of a specific sub-sequence.