Isothermal amplification

Patent Granted Some DNA amplification protocols have been developed that may be used alternatively to PCR: Helicase-dependent amplification is similar to traditional PCR, but uses a constant temperature rather than cycling through denaturation and annealing/extension steps. DNA Helicase, an enzyme that unwinds DNA, is used in place of thermal denaturation.[17] PAN-AC also uses isothermal conditions for amplification, and may be used to analyze living cells.[18][19] Nicking Enzyme Amplification Reaction referred to as NEAR, is isothermal, replicating DNA at a constant temperature using a polymerase and nicking enzyme. Recombinase Polymerase Amplification (RPA).