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Patent Granted To some extent, linear and 2-photon absorption strengths are linked. Therefore, the first compounds to be studied (and many that are still studied and used in e.g. 2-photon microscopy) were standard dyes. In particular, laser dyes were used, since these have good photostability characteristics. However, these dyes tend to have 2-photon cross-sections of the order of 0.1-10 GM, much less than is required to allow simple experiments.
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