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Patent Granted Primer extension is a technique whereby the 5' ends of RNA can be mapped. Primer extension can be used to determine the start site of RNA transcription (the end site cannot be determined by this method) by which its sequence is known. This technique requires a radiolabelled primer (usually 20 - 50 nucleotides in length) which is complementary to a region near the 3' end of the mRNA.
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