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Patent Pending Subtilisin is a non-specific protease (a protein-digesting enzyme) initially obtained from Bacillus subtilis.[2][3][4][5][6][7] Subtilisins belong to subtilases, a group of serine proteases that - like all serine proteases - initiate the nucleophilic attack on the peptide (amide) bond through a serine residue at the active site. Subtilisins typically have molecular weights of about 20,000 to 45,000 dalton.