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Patent Granted Plastic as a substrate. Disposable plastics are cheaper substrate as they are commonly made up of polystyrene. After use they should be disposed of properly. Before use they are treated with gamma radiation or electric arc simply to develop charges on the surface of substrate. After cell growth its rate of proliferation should be measured. In addition, the other plastic materials used as substrate are teflon or polytetrafluoroethylene (PTFE), thermamox (TPX), polyvinylchloride (PVC), polycarbonate, etc.