Type II restriction endonuclease

Patent Pending Typical type II restriction enzymes differ from type I restriction enzymes in several ways. They are a homodimer, with recognition sites are usually undivided and palindromic and 4–8 nucleotides in length. They recognize and cleave DNA at the same site, and they do not use ATP or AdoMet for their activity—they usually require only Mg2+ as a cofactor.[27] These are the most commonly available and used restriction enzymes.