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特許 係属中 PROBLEM TO BE SOLVED: To provide an authentication method using DNA capable of carrying out accurate authentication in a short time without influence of forgery by a person to be an authentication target. ; SOLUTION: In the authentication method using DNA, before authentication, appearance information of a one base polymorphic base sequence in a chromosome of the particular person to be the authentication target is recorded beforehand as information for collating in a storage medium isolated from the particular person.