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Patent Granted A microbial fuel cell (MFC) or biological fuel cell is a bio-electrochemical system that drives a current by using bacteria and mimicking bacterial interactions found in nature. MFCs can be grouped into two general categories, those that use a mediator and those that are mediator-less. The first MFCs, demonstrated in the early 20th century, used a mediator: a chemical that transfers electrons from the bacteria in the cell to the anode.